This dataset includes airborne in situ measurements of greenhouse gas mixing ratios, meteorological parameters, and fluxes (CO2, CH4, latent heat fluxes, friction velocity, and convective velocity scale) calculated with wavelet transforms. CO2, CH4, CO, O3, and water vapor mixing ratios, and meteorological variables were obtained from a Beechcraft A90 King Air aircraft. Flights occurred on April 19-26 2022, October 14-20 2022, February 5-13 2023, and April 13-19 2023 as part of the BlueFlux campaign, funded by NASA's Carbon Monitoring System program. Measurements were made with several instruments, including a PICARRO 2401-m (0.5 Hz CO2/CH4/H2O/CO), PICARRO 2311-f (10 Hz CO2/CH4/H2O), NASA Rapid Ozone Experiment (ROZE, 10 Hz O3), and AIMMS-20 probe (3-D winds, meteorology, and aircraft location data). Flight lines span Everglades National Park (ENP) and Big Cypress National Preserve (BCNP) in southern Florida, USA. The measurements were used to calculate vertical fluxes of trace gases and heat via wavelet transform eddy covariance