This dataset contains vegetation plot survey data collected at 36 sites across the Berg and Eerste River catchments in the Western Cape, South Africa collected during 2022 and again in 2023 during the 2023 BioSCape Field Collection season. Sampling protocols were designed by the BioSCape Leadership team for use by multiple teams to generate standardized and comparable vegetation plot data across sites. Each plot was circular, 10 m in diameter, and representative of the vegetation community. Species were identified at each 1-m mark along the N-S and W-E transects, totaling 42 identifications per plot. Fractional ground cover of the plot, fractional vegetation dominance, and rarefaction counts for each plot were recorded at all plots from 2023. Data from 2022 have fractional ground cover of the NE quadrant and fractional vegetation dominance. These data are provided in comma separated values (CSV) format. The boundaries of the patches where the vegetation plots were located were recorded as polygons in included Keyhole Markup Language (KML) files.