This dataset contains estimates for aboveground shrub biomass and uncertainty at high spatial resolution (0.80-m) across three research areas near Toolik Lake, Alaska. The estimates for August of 2013 were generated and mapped using Random Forest modeling with input variables of optimized LiDAR-derived canopy volume and height, mean NDVI from 4-band RGB color and near-IR orthophotographs, and harvested biomass data. Uncertainty in the final shrub biomass maps was quantified by producing separate maps showing the coefficient of variation (CV) of the Random Forest map estimates. Shrub biomass was harvested at Toolik Lake in 2014 and used to optimize inputs and validate the final model and these biomass data are also provided.